2301
Abstract Views
3934
PDF Download
Medical Laboratory Technology

Media Agar Tepung Kacang Hijau, Kacang Merah, Kacang Tunggak, Kacang Kedelai Sebagai Media Kultur Jamur Aspergillus Flavus

,
Pages 23-32

Abstract

Sabouraud Dextrose Agar (SDA) is a medium for the growth of fungi, ready to use, packaged in 500 gr, expensive, hard to find and takes a long time to obtain so it needs alternative media. SDA contains 4% glucose. The use of mung bean, red bean, cowpea and soy bean flour as culture media has never been done. The material contains high protein and high vegetable oil, allowing Aspergillusflavus to grow in it. The research aimed to know whether mung bean , red bean, cowpea, soy bean flour agar media may be used as culture media on the growth of Aspergillus flavus, and to know the diameter and color of colonies. Subjects were Aspergillusflavus fungus, inoculated in SDA media with incubation time 24 hours. Macroscopic shape: filamentous colonies, clear. Microscopic: insulated hyphae, branching, there are vesicles with conidia arranged like a fan. The object of the research are mung bean, red bean, cowpea, and soybean flour obtained from the manufacture of good quality peanut flour, whole seeds, not rancid, not wormy. Method: This research is an experimental study. The treatment was given to the independent variable and then measures the dependent variable. The result shows that mung bean, red bean, cowpea, soy bean flour agar media can be used as culture media on the growth of Aspergillusflavus fungus. Diameter of colony growth to five days on soybean flour agar medium was 7.1 cm, it was  greater than red beans media 6.1 cm, mung bean media  6.7 cm and SDA 6.5 cm. Colony color on all media are white, turns yellow and then green and dark green until the end. It can be concluded that Aspergillus flavus fungus can grow on mung bean, red bean, cowpea, soy bean flour agar media with various diameters.

There is no Figure or data content available for this article

References

1. Nurhayati. 2011. Penggunaan Jamur dan Bakteri dalam Pengendalian Penyakit Tanaman secara Hayati yang Ramah Lingkungan, Skripsi. Sumatera Selatan : Jurusan Hama dan Penyakit Tumbuhan, Fakultas Pertanian Universitas Sriwijaya Kampus Unsri,
2. Gandjar, Indrawati., Wellyzar Sjamsuridzal., Ariyani Oetari. 2006. Mikologi Dasar dan Terapan. Jakarta : Yayasan Obor Indonesia.
3. Tournas, V., M.E. Stack, P.B. Mislivec, and H.A. Koch. 2001. Yeast, Molds, and Mycotoxins. Washington :D.C Press.
4. Maryam, R. 2002. Mewaspadai Bahaya Kontaminasi Mikotoksin Pada Makanan. Falsafah Sains. Bogor : Program Pascasarjana, Institut Pertanian Bogor.
5. Handajani, N.S, dan R. Setyaningsih.2006. Identifikasi Jamur dan Deteksi Aflatoksin B1 terhadap Petis Udang Komersial. Jakarta. Biodiversitas.
6. Koneman, E.W , Lippincott Williams , Wilkins.2006. Koneman's Color Atlas and Textbook of Diagnostic Microbiology. Washington : Val Oduenyi Press.
7. Gandahusada, Srisasi, H. Herry D.Ilahude, Gita Pribadi. 2006.Parasitologi Kedokteran. Edisi 3. Jakarta: BalaiPenerbit FKUI.
8. Syarief, R., Ega, L., Nurwitri, C.,Mikotoksin Bahan Pangan,IPB Press, Bogor, 2003
9. Moore-Landecker, E. 1996. Fundamentals of the fungi. , E. 1996. Fundamentals of the fungi. 4th edition. Prentice Hall International, Inc., New Jersey, pp 576.
10. Bridson. 2006. Oxoid Microbiology. England : Oxoid limited.
11. Persatuan Ahli Gizi Indonesia. 2009. Tabel Komposisi Pangan Indonesia. Jakarta: PT. Elex Media Komputindo
12. Barton, R. C. 2013. Laboratory Diagnosis of Invasive Aspergillosis: From Diagnosis to Prediction of Outcome. Jurnal. Amerika : Department of Microbiology.
13. Pelczar, M.J., Chan, E.C.S. 2007. Dasar-dasar mikrobiologi. Jilid ke-1. Hadioetomo, R. S. , Imas, T., Tjitrosomo, S. S., Angka, S. L., penerjemah. Jakarta: UI Press. Terjemahan dari: Elements of Microbiology.
14. Ningrum, N. R. 2013. Analisis Pertumbuhan Jamur Aspergillus fumigatus dalam Media Kacang Hijau (Phaseolus radiatus L.). Karya Tulis Ilmiah. Yogyakarta : Jurusan Analis Kesehatan Stikes Jenderal Achmad Yani.
15. Panji. 2014. Jenis Karbohidrat Berdasarkan Jumlah Molekul Gulanya. Diunduh tanggal 26 Juni 2015 dari http://edubio.info.
16. Huwaina, A. D. 2015. Efektivitas Berbagai Konsentrasi Kacang Kedelai (Glycine max (L.) Merill) Sebagai Media Alternatif Terhadap Pertumbuhan Jamur Candida albicans. Karya Tulis Ilmiah. Yogyakarta : Jurusan Analis Kesehatan Poltekkes Kemenkes Yogyakarta.
There is no Supplemental content for this article.

How to Cite This

Nuryati, A., & Sujono, S. (2017). Media Agar Tepung Kacang Hijau, Kacang Merah, Kacang Tunggak, Kacang Kedelai Sebagai Media Kultur Jamur Aspergillus Flavus. Jurnal Teknologi Kesehatan (Journal of Health Technology), 13(1), 23–32. Retrieved from https://e-journal.poltekkesjogja.ac.id/index.php/JTK/article/view/13

Article Metrics

Download Statistics

Downloads

Download data is not yet available.

Other Statistics

Verify authenticity via CrossMark

Copyright and Permissions

Publishing your paper with Jurnal Teknologi Kesehatan (Jurtekkes) means that the author or authors retain the copyright in the paper. Jurtekkes granted an exclusive reuse license by the author(s), but the author(s) are able to put the paper onto a website, distribute it to colleagues, give it to students, use it in your thesis etc, even commercially. The author(s) can reuse the figures and tables and other information contained in their paper published by Jurtekkes in future papers or work without having to ask anyone for permission, provided that the figures, tables or other information that is included in the new paper or work properly references the published paper as the source of the figures, tables or other information, and the new paper or work is not direct at private monetary gain or commercial advantage.

Jurtekkes journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. This journal is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License. This license lets others remix, transform, and build upon the material for any purpose, even commercially.

Jurtekkes journal Open Access articles are distributed under this Creative Commons Attribution-ShareAlike 4.0 International License (CC BY-SA). Articles can be read and shared for All purposes under the following conditions:

  • BY: You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
  • SA:  If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.

Data Availability

 

visitorstatistic

WEBSITE VISITORS

Flag Counter

View My Stats