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Medical Laboratory Technology

Perbedaan Kadar Kreatinin Pada Serum Lipemik Yang Diolah Dengan Polyethylene Glycol 6000 8% Dan High Speed Sentrifugasi

Wheny Mufita Sari Jurusan Analis Kesehatan Poltekkes Kemenkes Yogyakarta, Indonesia Correspondence whenymufita@gmail.com
,
Ni Ratih Hardisari Jurusan Analis Kesehatan Poltekkes Kemenkes Yogyakarta, Indonesia Correspondence ratihhardisari@gmail.com
,
Sujono Sujono Jurusan Analis Kesehatan Poltekkes Kemenkes Yogyakarta, Indonesia Correspondence sujono_analis@yahoo.co.id
Pages 45-49
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Abstract

Lipemic serum is a turbid serum caused by enhancement of lipoprotein consentration mainly chylomicron and very low density lipoprotein (VLDL). It can cause interference of physics and chemistry, spectrofotometri, not homogen sample and volume displacement effect that make the result of the laboratory test inacurate, including creatinin test. The method that can be used to clear up lipid on the lipemic serum is centrifugation, extraction with polar solvent, dilution, and precipitation with Polyethylene Glycol 6000 8%. The aim of the study is to know creatinine level defference of lipemic serum using Polyethylene Glycol 6000 8% in creatinine test. This research was a true experiment with Pretest Postest Control Group Design. This research was conducted in October – November 2016 at Pathology Clinic of Panembahan Senopati Hospital, Bantul. The result of creatinine scores before treatments was 0,98 mg/dL, after being added with Polyethylene Glycol 6000 8% it became 0,98 mg/dL, and after being processed using High Speed Centrifugation, it was 1,11 mg/dL. The result of statistic test using Independent Sample T-Test obtained the Asymp sig value of 0,003 (p<0,05) which means there is no difference of creatinine scores in lipemic serum processed by adding Polyethylene Glycol 6000 8% and High Speed Centrifugation.

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References

1. WHO. (2002). Use of Anticoagulants In Diagnostic Laboratory Invertigation.
2. Nikolac, N. (2013). Lipemia: Causes, Interference Mechanisms, Detection and Manajement. Biochemia Medica 2014;24(1):56-57. Kroasia : University Departement of Chemistry.
3. Contois, J.H dan Nguyen, R.A. (2013). Assay Interference: A Need for Increased Understanding and Testing. Sun Diagnostic.
4. Kee, M. (2013). Pedoman Pemeriksaan Laboratorium dan Diagnostik Edisi 6. Jakarta: ECG Penerbit Buku Kedokteran.
5. Sacher, R.A. dan McPherson (2004). Tinjauan Klinis Hasil Pemeriksaan Laboratorium. Jakarta : EGC
6. Sugiyono. (2010). Statistika Untuk Penelitian. Edisi 2. Bandung : Alfabeta.
7. Saracevic, A., Nikolac, N., Simundic, A.M. (January, 2014). The Ealuation and Comparation of Consecutive High Spees Centrifugation and LipClear Reagent for Lipemia Removal. Clinical Chemistry, Vol. 47, 309-314.
8. Calmarza P. dan Cordero J. (2011, May 8). Lipemia Interferences in Routine Clinical Biochemiae Tests. Biochemia Medica 2011;21(2):160-6.
9. Dimeski , G., Jones, B.W. 2011. Lipaemic samples : Evvective Process for Lipid Reduction Using High Speed Centrifugation Compared With Ultracentrifugation. Biochemia Medica 200; 21(1):86-94.
10. Guder, W. G dan Sheshadri N. (2015). Pre-Examination Procedures in Laboratory Diagnostics. Germany: De Gruyter.
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How to Cite This

Sari, W. M., Hardisari, N. R., & Sujono, S. (2017). Perbedaan Kadar Kreatinin Pada Serum Lipemik Yang Diolah Dengan Polyethylene Glycol 6000 8% Dan High Speed Sentrifugasi. Jurnal Teknologi Kesehatan (Journal of Health Technology), 13(1), 45–49. Retrieved from https://e-journal.poltekkesjogja.ac.id/index.php/JTK/article/view/21

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