Perbedaan jarak sinar Ultraviolet-C terhadap penurunan bakteri Coliform pada air bersih di dapur PT M
DOI:
https://doi.org/10.29238/sanitasi.v17i1.2524Keywords:
Air bersih, Coliform, Jarak Penyinaran, Ultraviolet-CAbstract
Ditemukannya bakteri Coliform sebesar 107 CFU/100ml pada air bersih di dapur PT M yang tidak memenuhi standar baku mutu dalam Peraturan Menteri Kesehatan No. 2 tahun 2023 yaitu 0 CFU/100ml. Tujuan penelitian ini untuk mengetahui jumlah dan penurunan bakteri Coliform sebelum dan sesudah desinfeksi sinar ultraviolet-C dengan perbedaan jarak sinar 2 cm, 5 cm, 8 cm dan jarak sinar ultraviolet-C yang efektif menurunkan bakteri Coliform. Penelitian ini dilakukan pada bulan Maret – Mei 2024 dengan jenis penelitian eksperimen dalam skala lapangan dan menggunakan metode kuantitatif dengan desain penelitian posttest with control. Besar sampel yang digunakan sebanyak 24 sampel mikrobiologi air bersih yang diperiksa di Laboratorium. Hasil pengukuran fisik air bersih di lapangan menunjukkan bahwa suhu, pH, TDS, dan kekeruhan sudah memenuhi standar sehingga tidak mempengaruhi atau mengurangi efektivitas sinar ultraviolet-C. Desinfeksi sinar ultraviolet-C terhadap penurunan bakteri Coliform pada air bersih dengan perbedaan jarak 2 cm, 5 cm, dan 8 cm selama 30 detik dengan daya 30 watt dan kedalaman air 5 cm menunjukkan hasil penurunan yang signifikan. Paparan sinar ultraviolet-C dengan jarak sebesar 2 cm paling efektif dalam menurunkan bakteri Colifrom sebesar 100% karena semakin dekat jarak sinar ultraviolet-C, maka semakin cepat bakteri kehilangan patogenesisnya.
Abstract: The detection of Coliform bacteria at 107 CFU/100ml in the clean water in PT M's kitchen does not meet the quality standard set by the Minister of Health Regulation No. 2 of 2023, which is 0 CFU/100ml. This study aims to assess the quantity and reduction of Coliform bacteria before and after ultraviolet-C (UV-C) disinfection at distances of 2 cm, 5 cm, and 8 cm, and to identify the most effective UV-C distance for reducing Coliform bacteria. Conducted from March to May 2024, the research used an experimental field-scale quantitative method with a posttest control design. A total of 24 clean water microbiological samples were tested in the laboratory. Field measurements of clean water showed that temperature, pH, TDS, and turbidity met the standards, not affecting UV-C effectiveness. UV-C disinfection for reducing Coliform bacteria in clean water at distances of 2 cm, 5 cm, and 8 cm for 30 seconds with a power of 30 watts and a water depth of 5 cm showed significant reductions. UV-C exposure at a distance of 2 cm was the most effective, reducing Coliform bacteria by 100%, as closer UV-C light distances accelerate the loss of bacterial pathogenicity.
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Copyright (c) 2024 May Iasa Badrianti, Ujang Nurjaman, Lubis Bambang Purnama, Payzar Wahyudi

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